Exploring the Antioxidant Power of Plant Extracts in 2018 The ABTS Assay Insights
In 2018, a significant study was conducted to evaluate the antioxidant activity of various plant extracts using the ABTS assay. This method is a powerful tool for assessing the ability of compounds to neutralize free radicals, which are known to cause oxidative stress and contribute to the aging process and various diseases.
Understanding the ABTS Assay
The ABTS assay, also known as the 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay, is widely used for quantifying the antioxidant capacity of samples. It measures the reduction of 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by antioxidants in a test sample.
Selection of Plant Extracts
For the study, a diverse range of plant extracts was selected, including those from fruits, herbs, and spices. These extracts were chosen based on their traditional use and historical significance in antioxidant properties.
Methodology
The experimental procedure involved the preparation of stock solutions of the plant extracts and subsequent dilutions to achieve a range of concentrations. The ABTS solution was prepared and its absorbance was measured at 734 nm. The extracts were then added to the ABTS solution, and the absorbance was measured again after incubation. The decrease in absorbance indicated the antioxidant activity of the extracts.
Results and Analysis
The results showed significant variations in antioxidant activity among the different plant extracts. Some extracts, such as those from green tea and pomegranate, demonstrated exceptionally high antioxidant power. This finding aligns with previous studies on the health benefits of these plants.
Conclusion
The ABTS assay provided valuable insights into the antioxidant properties of plant extracts, highlighting the potential of natural sources in combating oxidative stress. This research not only contributes to scientific knowledge but also has practical applications in the development of health supplements and functional foods.