Pfu DNA Polymerase
Specification:100 U, 500 U
Concentration:100 U--2,500 U/ml, 500 U--2,500 U/ml
Product description:Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5¨★3¨ direction. Pfu DNA Polymerase also exhibits 3¨★5¨ exonuclease (proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5¨★3¨ exonuclease activity.
1.High fidelity PCR
2.Generation of PCR products for cloning and expression
3.RT-PCR for cDNA cloning and expression
4.Blunt-end PCR cloning
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