T4 Polynucleotide Kinase
Product description:T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible. In the presence of ADP T4 Polynucleotide Kinase exhibits 5'-phosphatase activity and catalyzes the exchange of phosphate groups between 5'-P-oligo-polynucleotides and ATP (exchange reaction)
1.Labeling of nucleic acids' 5'-termini to be used as (see Figure 1 in Supporting Data):
probes for hybridization
probes for transcript mapping
markers for gel electrophoresis
primers for DNA sequencing
primers for PCR
2.5'-phosphorylation of oligonucleotide, PCR products, other DNA or RNA prior to ligation
3.Phosphorylation of PCR primers
4.Detection of DNA modification by the [32P]-postlabeling assay
5.Removal of 3'-phosphate groups(see? Reference2)
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