T4 RNA Ligase
Specification:500 U, 5,000 U
Product description:T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.
The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.
1.RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate
2.Joining RNA to RNA
3.Synthesis of oligoribonucleotides and oligodeoxyribonucleotides
4.Specific modifications of tRNAs
5.Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5' RACE (Rapid Amplification of cDNA Ends)
6.Site-specific generation of composite primers for PCR
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