Phi29 DNA Polymerase

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Phi29 DNA Polymerase

Specification:250 U, 1,250 U
Concentration:250 U---10,000 U/ml, 1,250 U---10,000 U/ml
Product description:phi29 DNA Polymerase is a highly processive polymerase (up to more than 70 kb) featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. phi29 DNA Polymerase also possesses a 3'★5' exonuclease (proofreading) activity acting preferentially on single-stranded DNA or RNA (see Reference 3). Therefore 3'-modified primers are highly recommended.
Product application:
1.Rolling circle amplification (RCA) (see Reference 6): generation of periodic DNA nanotemplates.
2.Multiple displacement amplification (MDA).
3.DNA template preparation for sequencing.
 

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